Biology Lab Report on the extraction of Chlorophyl from Plant Leaves

Abstract

This experiment focused on extracting and separating pigments of Chloroplast. For the procedure grand leaves were grinded in a mortar with some chemicals and the fluid was filtrated to use for make headway analysis. Stripes of this solution were put on a tense paper and later, after dried placed into a beam of solvent. After this the chloroplast pigments were separated by the solvent into groups of more or less soluble pigments.

Aim

How many pigment types are accede in a green leaf?

It is hoped to be fit to identify the four different pigments types of a leaf. As the filter paper with the solvent will separate the pigments in basis of solubility, a clear segmentation of individually is expected to turn up off.

As various chemicals were used in the whole process of this lab, reliable variables might have influenced the results in terms of the purity of each chemical or purity of the used filtrate.

Background

The chloroplast, basically, is the organelle trusty for photosynthesis. Structurally it is very similar to the mitochondrion. It contains a permeable outer(prenominal) membrane, a less permeable intragroup membrane, a intermembrane space, and an inner section called the stroma. However, the chloroplast is larger than the mitochondria. It needs to have the larger coat because its membrane is not folded into cristae. Also the inner membrane is not used for the electron shipping chain.



Instead it contains the light-absorbing system, the electron transport chain, and ATP synthetase in a third membrane that forms a series of flattened discs, called the thylakoids.

Materials required

Materials list

Test tubes, Water, small beacon, Pipette, Stand, Mortar, green leaves, Filter paper, capillary tube tube, funnel, silicon dioxide, calcium carbonate, acetone, solvent.

Method

1) To extract pigments from green Leaves

- Weigh 5g green Leaves, cut into pieces, and...

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